Title

Direct DNA Binding Activity of the Fanconi Anemia D2 Protein

Document Type

Article

Publication Date

4-23-2005

DOI

10.1074/jbc.M503730200

Version

Citation

Format

Other

Publication Title

Journal of Biological Chemistry

Keywords

Fanconi anemia D2 protein, FANCD2, Fanconi anemia, DNA, genetics

Subject Categories

Biology

Abstract

It is known that the Fanconi anemia D2 protein is vital for protecting the genome from DNA damage, but what activities this protein has are unknown. In these experiments we purified full-length Fanconi anemia protein D2 (FANCD2), and we found that FANCD2 bound to DNA with specificity for certain structures: double strand DNA ends and Holliday junctions. Proteins containing patient-derived mutations or artificial variants of the FANCD2 protein were similarly expressed and purified, and each variant bound to the Holliday junction DNA with similar affinity as did the wild-type protein. There was no single discrete domain of FANCD2 protein that bound to DNA, but rather the full-length protein was required for structure-specific DNA binding. This finding of DNA binding is the first biochemical activity identified for this key protein in the Fanconi anemia pathway.