Document Type

Article

Publication Date

1994

Version

Post-print

Format

PDF

Publication Title

Journal of Biological Chemistry

Keywords

Polymerase Chain Reaction, PCR, spider silk, spiders, amino acids

Abstract

The Polymerase Chain Reaction (PCR) has been used to amplify the portion of the Spidroin 1 gene that codes for the C-terminal part of the silk protein of the spider Nephila clavipes. Along with some substitution mutations of minor consequence, the PCR-derived sequence reveals an additional base missing from the previously published Nephila Spidroin 1 sequence. Comparison of the PCR-derived sequence with the equivalent region of Spidroin 2 indicates that the insertion of this single base results in greatly increased similarity in the resulting amino acid sequences of Spidroin 1 and Spidroin 2 (75% over 97 amino acids). The same PCR primers also amplified a fragment of the same length from Araneus bicentenarius. This sequence is also very similar to Spidroin 1 of Nephila (71% over 238 bases excluding the PCR primers, which translates into 76% over 79 amino acids).

Comments

This is the author's post-print of research originally published in the Journal of Biological Chemistry 269:6661-6663, © 1994, the American Society for Biochemistry and Molecular Biology.

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