Title

Developmental Sex Differences in Glutamic Acid Decarboxylase (GAD65) and the Housekeeping Gene, GAPDH

Document Type

Article

Publication Date

12-2001

DOI

10.1016/S0006-8993(01)03167-5

Version

Citation

Publication Title

Brain Research

Keywords

GABAergic system, sexual differentiation, rat brain, developmental sex differences, GAD65 protein

Subject Categories

Biology

Abstract

Previous work has demonstrated that the GABAergic system is involved in sexual differentiation of the rodent hypothalamus. The present study was designed to further examine this involvement by investigating developmental sex differences in GAD65 protein levels in hypothalamic and extrahypothalamic brain regions known to be sexually dimorphic in adulthood. Brain nuclei were micro-dissected and GAD65 protein levels were quantified using western immunoblotting. Sex differences in levels of GAD65 were found in the dorsomedial nucleus and preoptic area of the hypothalamus and also the medial amygdaloid nucleus and CA1 subfield of the hippocampus. Unexpectedly, there were sex differences in protein levels of the housekeeping gene, GAPDH, cautioning against the use of GAPDH for standardizing protein samples during western immunoblotting.